POINT OF CONTACT - doerc100005

ERDC TN-DOER-C10

March 2000

of the sediments at a series of reaches away from the point source will be analyzed by both chemical

analysis and cell-based assays. The results of this study will be reported in a future DOER Technical

Note.

POINT OF CONTACT: For additional information, contact Dr. Laura S. Inouye (601-634-2910,

inouyel@wes.army.mil), Dr. Victor A. McFarland (601-634-3721, mcfarlv@wes.army.mil), or the

Manager of the Dredging Operations Environmental Research Program, Dr. Robert M. Engler

(601-634-3624, englerr@wes.army.mil).

Ang, C. Y., Inouye, L. S., McCant, D. D., and McFarland, V. A. (2000). "Protocols for

a rapid clean-up/extraction procedure and an improved P450RGS Dioxin Screening

Assay for Sediments," DOER Technical Notes Collection (ERDC TN-DOER-C10), U.S.

Army Engineer Research and Development Center, Vicksburg, MS.

www.wes.army.mil/el/dots/doer

REFERENCES

Anderson, J. W., Zeng, E. Y., and Jones, J. M. (1999). "Correlation between response of human cell line and

distribution of sediment polycyclic aromatic hydrocarbons and polychlorinated biphenyls on Palos Verdes Shelf,

California, USA," Environmental Toxicology and Chemistry 18, 1506-1510.

Anderson, J. W., Rossi, S. S., Tukey, R. H., Vu, T., amd Quattrochi, L. C. (1995). "A biomarker, P450RGS, for

assessing the potential toxicity of organic compounds in environmental samples," Environmental Toxicology and

Chemistry 14, 1159-1169.

Anderson, J. W., Newton, F. C., Hardin, J., Tukey, R. H., and Richter, K. E. (1996). "Chemistry and toxicity of

sediments from San Diego Bay, including a biomarker (P450RGS) response." Environmental Toxicology and Risk

Assessment: Biomarkers and Risk Assessment, ASTM STP 1306, A. D. Bengston and D. S. Henshel, eds., American

Society for Testing and Materials, West Conshohocken, PA, 5, 53-78.

Bradlaw, J. A., and Casterline, J. L., Jr. (1979). "Induction of enzyme activity in cell culture: A rapid screen for detection

of planar polychlorinated organic compounds," Journal of the Association of Official Analytical Chemists 62,

904-916.

Hoke, R. A., Jones, P. D., Maccubbin, A. E., Zabik, M. J., and Giesy, J. P. (1994). "Use of in vitro microbial assays

of sediment extracts to detect and quantify contaminants with similar modes of action," Chemosphere 28, 169-181.

Kim, G. B., Anderson, J. W., Bothner, K., Lee, J-H., Koh, C-H., and Tanabe, S. (1997). "Application of P450RGS

(reporter gene system) as a bioindicator of sediment PAH contamination in the vicinity of Inchon Harbor, Korea,"

Biomarkers 2, 181-188.

McCant, D. D., Inouye, L. S., and McFarland, V. A. (1999). "A One-Step ASETM extraction method for TCDD TEQ

determination," Bulletin of Environmental Contamination and Toxicology 63, 282-288.

McFarland, V. A., McCant, D. D., and Inouye, L. S. (1998). "Guidance for performance of the H4IIE dioxin screening

assay," DOER Technical Notes Collection (TN DOER-C1), U.S. Army Engineer Waterways Experiment Station,

Vicksburg, MS. www.wes.army.mil/el/dots/doer

McFarland, V. A., Ang, C. Y., McCant, D. D., Inouye, L. S., and Jarvis, A. S. (1999). "Comparison of two cell-based

assays for screening dioxin and dioxin-like compounds in sediments," DOER Technical Notes Collection (TN

DOER-C8), U.S. Army Engineer Research and Development Center, Vicksburg, MS. www.wes.army.mil/

el/dots/doer

Murk, A. J., Legler, J., Denison, M. S., Giesy, J. P., van de Guchte, C., and Brouwer, A. (1996). "Chemical-activated

luciferase gene expression (calux): A novel in vitro bioassay for AH receptor active compounds in sediments and

pore water," Fundamental and Applied Toxicology 33, 149-160.