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Page Title: SOLVENT EXCHANGE
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ERDC TN-DOER-C10
March 2000
3) Transfer the sample to a 0.3-mL conical insert marked at the 100-L level.
4) Rinse the Zymark tube with 80 L iso-octane and transfer rinse to the conical
insert.
5) Using the MiniVap (nitrogen evaporator), gently blow down the extract to the
100-L mark.
6) Place the conical insert into a 4-mL amber glass vial with Teflon-lined cap for
storage in a -4 C freezer. The conical insert minimizes evaporative loss by
reducing both the head space over the sample in the storage vial and the surface
area exposed when the vial is opened.
11

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