Table 1. Contaminant Dosing for HepG2 Cells (Nominal Concentrations)

ERDC TN-DOER-R4

September 2004

Table 1

Contaminant Dosing for HepG2 Cells (Nominal Concentrations)

PM180 Mixture Component list

High PAH (ug/L)

Mid PAH (ug/L)

Low PAH (ug/L)

acenaphthene

251

25.1

2.5

acenaphthylene

251

25.1

2.5

anthracene

251

25.1

2.5

benz[a]anthracene

2.5

0.3

Benzo[b]fluoranthene

2.5

0.3

Benzo[k]fluoranthene

1.3

0.1

Benzo[ghi]perylene

2.5

0.3

Benzo[a]pyrene

2.5

0.3

chrysene

2.5

0.3

dibenz[a,h]anthracene

2.5

0.3

fluoranthene

2.5

0.3

fluorene

251

25.1

2.5

indeno[1,2,3-cd]pyrene

2.5

0.3

naphthalene

251

25.1

2.5

phenanthrene

251

25.1

2.5

pyrene

2.5

0.3

SUM PAH

1740 ug/L

174.0 ug/L

17.4 ug/L

Aroclor 1254 (C12H(10-x)Clx)

High Aroclor (ug/L)

Mid Aroclor (ug/L)

Low Aroclor (ug/L)

x=2

0.24

0.024

0.0024

x=3

1.26

0.126

0.0126

x=4

10.25

1.025

0.1025

x=5

59.12

5.912

0.5912

x=6

26.76

2.676

0.2676

x=7

2.66

0.266

0.0266

x=8

0.04

0.004

0.0004

x=9

0.04

0.004

0.0004

SUM PCBs

100 ug/L

10 ug/L

1 ug/L

High TCDD

Mid TCDD

Low TCDD

2,3,7,8-TCDD

100 ng/L (311 pM)

25 ng/L (78 pM)

7.5 ng/L (23 pM)

Aroclor1254 components are listed by the number of chlorines rather than the individual polychlorinated biphenyl congeners.

The data were then analyzed using a semi-parametric technique that uses local regression to

estimate the normalized expression levels as well as the expression level-dependent error

variance (Kepler et al. 2002). The technique is based on assumptions that the large majority of

genes will not have their relative expression levels changed from one treatment group to the next,

and that departures of the response from linearity are small and vary slowly. For each compound,

gene responses at each dose were compared to controls independently. The statistical data and

gene expression data were then examined to determine which genes were responding

consistently across all doses.