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 G.3.2.2 Chemical analysis ................................................................G6
G.3.3 Data presentation and analysis ......................................................G6
G.4 References ..............................................................................................G7
Appendix H: Plant Bioaccumulation Procedures................................................H1
H.1 Introduction ............................................................................................H1
H.2 DTPA Extraction Procedure for Plant Bioaccumulation ........................H1
H.2.1 Materials .......................................................................................H2
H.2.2 Sediment preparation ....................................................................H2
H.2.3 Sediment characterization .............................................................H2
H.2.4 DTPA extraction procedure ..........................................................H3
H.2.5 Prediction of plant bioaccumulation and comparison
to reference.......................................................................................H3
H.2.6 Comparison of DTPA results to reference ....................................H4
H.3 Laboratory Plant Bioaccumulation Procedures.......................................H5
H.3.1 Plant bioaccumulation/toxicity assessment ...................................H5
H.3.2 Apparatus and materials................................................................H5
H.3.3 Sediment collection and preparation .............................................H7
H.3.4 Sediment characterization .............................................................H7
H.3.5 Greenhouse operation and bioaccumulation techniques ...............H8
H.3.6 Plant tissue collection and preparation for analysis.......................H9
H.3.7 Data presentation and analysis ......................................................H9
H.4 References ..............................................................................................H9
Appendix I: Reserved .......................................................................................... I1
Appendix J: Reserved .......................................................................................... J1
Appendix K: Reserved........................................................................................K1
Appendix L: Statistical Methods ........................................................................ L1
L.1 Introduction ............................................................................................ L1
L.1.1 Basic statistics ............................................................................... L3
L.1.2 Hypothesis testing ......................................................................... L6
L.1.3 Experimental design ...................................................................... L8
L.2 Statistical Methods for Water Column Tests .......................................... L9
L.2.1 Water column toxicity tests ........................................................... L9
L.2.1.1 Comparison of 100 percent elutriate and dilution water ...... L9
L.2.1.1.1 Methods...................................................................... L9
L.2.1.1.2 Analysis of example data.......................................... L15
L.2.1.2 Calculating median lethal concentration ............................ L19
L.2.1.2.1 Methods for calculating LC50 ................................... L20
L.2.1.2.2 Analysis of example data.......................................... L24
L.2.2 Linear regression ......................................................................... L25
L.3 Statistical Methods for Bioaccumulation .............................................. L27
L.3.1 Methods for multiple comparisons .............................................. L27
L.3.2 Analysis of example data............................................................. L30
L.3.2.1 Comparisons with a reference sediment............................. L30
L.3.2.2 Comparison with an action level........................................ L36
L.3.3 Bioaccumulation from field data ................................................. L39
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