Appendix G: Animal Bioaccumulation Test Procedures

Appendix G

Animal Bioaccumulation Test

Procedures

G.1 Introduction

This appendix provides detailed step-by-step procedures for conducting tests

for evaluation of terrestrial animal bioaccumulation of contaminants. The

background, rationale, and tiered framework for application of these tests are

discussed in Chapter 8 of the main text of this Upland Testing Manual (UTM).

Two test procedures are included in this appendix:

a. Calculation of theoretical bioaccumulation potential for evaluation of

potential terrestrial animal bioaccumulation of nonpolar organic

contaminants.

b. Quantitative test for bioaccumulation of contaminants by terrestrial

animals as represented by the earthworm.

G.2 Tier II - Theoretical Bioaccumulation Potential

(TBP) Of Nonpolar Organic Chemicals

The TBP is an approximation of the equilibrium concentration in tissues if

the dredged material in question were the only source of contaminant to the

organisms. The TBP calculation in Tier II is applied as a screen to calculate the

magnitude of bioaccumulation likely to be associated with nonpolar organic

contaminants in the dredged material.

Nonpolar organic chemicals include all organic compounds that do not

dissociate or form ions. This includes the chlorinated hydrocarbon pesticides,

many other halogenated hydrocarbons, PCBs, many PAHs including all the COC

PAHs, dioxins, and furans. It does not include metals and metal compounds,

organic acids or salts, or organometallic complexes such as tributyltin or methyl

mercury.

Appendix G Animal Bioaccumulation Test Procedures