Washing/Purging the injectors A and C

ERDC TN-DOER-C10

March 2000

2) Washing/Purging the injectors A and C.

a) Click on Wash/Purge option and then both Injector A and C.

b) Click on Utility menu and then Plate Out option.

c) Place the drain plate in the mask door and close the mask door. Click on

Plate In option.

d) The dispenser will draw water (ultrapure water) from the dispenser tubes three

times to wash the injectors. After the injectors are washed, pour off the

contents of the drain plate and place the plate back into the mask door.

3) Priming the injectors.

a) Transfer Substrate A into a dispenser bottle (amber) and Substrate B into

another.

b) Connect dispenser A to the Substrate A bottle and dispenser C to the Substrate

B bottle.

c) Click on Prime Injector, followed by the Injector A. The injector will draw

Substrate A through three times. Injector A is now primed.

d) Pour off the contents of the drain plate and return the plate to the mask door.

e) Prime Injector C similarly for Substrate B.

Remove drain plate and click on Done.

g) The luminometer is now primed.

4) Retrieving the assay template

a) Click on the File menu.

b) Open a previously prepared file for the assay template, designed to read an

entire luminometer plate comprising 1 control background, 7 TCDD

standards, and 24 samples in triplicate wells as matching the plate dosing

template (see Section III, "DAY 3, DOSING THE CELLS").

c) Click on Settings and choose the Template option. (Take out the wells that

were preset in the assay template if fewer samples are tested for the day.)

d) Click on the Assay Option from the Settings menu. The numbers should

correspond with the numbers in the assay template. However, if fewer

samples are being tested for the day, the numbers can be changed

accordingly. These numbers should correspond to the number of wells

included in the Template option.

Reading the plate and printing the results.

a) Click on Run A Plate (the icon with a person facing the monitor) and take

off the Search for Sample ID.