Recovering Substrate A and B from the injectors

ERDC TN-DOER-C10

March 2000

b) Click on Plate ID and type the filename as datergs (e.g., August 1 as 213rgs)

for the day's experiment. If a second plate is run on the same day, type

datergs2, or for a third plate, then datergs3, and so on.

c) NOTE: The results will be automatically saved on the computer and can be

called up when required.

d) Click on Start. The luminometer will start reading. The injectors will inject

100 L of Substrate A and Substrate B into each well simultaneously.

e) Click on the Continue button to instruct the luminometer to read from one

well to another. There is a 2-second delay between reading the wells.

When all the wells are read, the Printer Menu will appear at the end of the run.

Click on Cancel (the entire report is very long) to avoid printing all the flash

data report.

g) Click on View Data Matrix and print the raw data in relative luminescence

unit (RLU).

h) Click on View Area Statistics. Print the page(s) as needed, usually 1 page

long. This report will show the average and other simple statistics for every

sample.

6) Recovering Substrate A and B from the injectors.

a) Click on Utility menu.

b) Place drain plate in the mask door.

c) Click on Dispensers and then Recover Injectors option.

d) Transfer any remaining Substrate A and Substrate B into their respective

storage tubes. Wrap the tube containing Substrate B with aluminum foil.

e) Store the substrates immediately in the -80 C freezer for later use.

Go through the wash/purge steps to clean the injector with ultrapure water.

7) Closing the template file.

a) Click on File followed by the Close button twice to close the View Area

Statistics and Data Matrix reports.

b) Click on File and Close again to close the assay template. If some changes

were made on the template, a screen will appear. It is not necessary to save

any changes made on the template.

8) Exiting the program.

a) Click on File and then on Exit to exit the Revelation program.

b) Click on File and then on Exit again to exit the Windows program. Click on

OK to get into the C:> prompt.

c) Turn off the luminometer switch.